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    • Rights: The University of Waikato
    Published 9 June 2011 Referencing Hub media
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    Dr Richard Espley

    Some plant species1 are not susceptible to Agrobacterium DNA2 integration, and in that case, we could use an alternative method for transforming a plant such as biolistics3.

    Biolistics is like a gun that shoots tiny gold4 particles that are coated in DNA into the plant, and if you shoot enough of these particles into the leaf, for example, of a plant, some of it will get integrated and you get transformed cells.

    © The University of Waikato

    Acknowledgements
    Calvin College, Michigan
    Bio-Rad Laboratories Pty Ltd

    1. species: (Abbreviation sp. or spp.) A division used in the Linnean system of classification or taxonomy. A group of living organisms that can interbreed to produce viable offspring.
    2. DNA: Deoxyribonucleic acid (DNA) is a molecule that contains the instructions needed for an organism to develop and function. These instructions are stored as a code made up of four chemical bases: adenine (A), guanine (G), cytosine (C) and thymine (T).
    3. biolistics: Using a particle bombardment system, like a gene gun, to deliver DNA into a cell.
    4. gold: A transition metal in Group 11 of the periodic table – symbol Au, atomic number 79.

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    INTERACTIVE

    Making a transgenic plant

    This interactive illustrates a technique used by scientists to generate transgenic plants.

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      species

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      2. (Abbreviation sp. or spp.) A division used in the Linnean system of classification or taxonomy. A group of living organisms that can interbreed to produce viable offspring.

      gold

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      3. A transition metal in Group 11 of the periodic table – symbol Au, atomic number 79.

      DNA

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      4. Deoxyribonucleic acid (DNA) is a molecule that contains the instructions needed for an organism to develop and function. These instructions are stored as a code made up of four chemical bases: adenine (A), guanine (G), cytosine (C) and thymine (T).

      biolistics

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      5. Using a particle bombardment system, like a gene gun, to deliver DNA into a cell.

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