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  • Information within a gene construct.
    Rights: The University of Waikato Published 16 March 2010 Size: 26 KB Referencing Hub media

    A gene1 construct contains all the information needed for transfection into a bovine cell2 and expression of the desired gene in a cow. This includes an antibiotic3 resistance4 marker5, a tissue-specific promoter6, the transgene/gene of interest and a stop sequence.

    1. genes: A segment of a DNA molecule that carries the information needed to make a specific protein. Genes determine the traits (phenotype) of the individual.
    2. cell: 1. Building block of the body. A human is made of millions of cells, which are adapted for different functions and can reproduce themselves exactly. 2. A simple electrolytic device that enables chemical energy to be transformed into electrical energy.
    3. antibiotic: A substance produced by an organism that kills bacteria.
    4. resistance: 1. The opposition to the flow of electric current through a circuit. 2. The ability to withstand harmful or unfavourable conditions, such as an infectious disease.
    5. marker: A short sequence of DNA within a genome that can be readily detected. Markers are usually associated with a known gene and provide information about which allele(s) of the gene an individual is likely to carry.
    6. promoter: A DNA sequence where RNA polymerase binds to begin transcription.
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      genes

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    2. A segment of a DNA molecule that carries the information needed to make a specific protein. Genes determine the traits (phenotype) of the individual.

      resistance

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    4. 1. The opposition to the flow of electric current through a circuit.

      2. The ability to withstand harmful or unfavourable conditions, such as an infectious disease.

      cell

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    6. 1. Building block of the body. A human is made of millions of cells, which are adapted for different functions and can reproduce themselves exactly.

      2. A simple electrolytic device that enables chemical energy to be transformed into electrical energy.

      marker

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    8. A short sequence of DNA within a genome that can be readily detected. Markers are usually associated with a known gene and provide information about which allele(s) of the gene an individual is likely to carry.

      antibiotic

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    10. A substance produced by an organism that kills bacteria.

      promoter

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    12. A DNA sequence where RNA polymerase binds to begin transcription.