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Timeline – History of microscopy

An interactive look at some of the key advances in microscopy. Find out more by browsing or searching the Science Learning Hub.

Acknowledgements
Image: Rebecca Campbell, University of Otago

~710 BC

Nimrud lens

The Nimrud lens – a piece of rock crystal – may have been used as a magnifying glass or as a burning-glass to start fires by concentrating sunlight. It is later unearthed by Austen Henry Layard at the Assyrian palace of Nimrud in modern-day Iraq.

~1000 AD

Reading stone

The first vision aid, called a reading stone, is invented. It is a glass sphere placed on top of text, which it magnifies to aid readability.

~1021 AD

Book of Optics

Muslim scholar Ibn al-Haytham writes hisBook of Optics. It eventually transforms how light and vision are understood.

1284

First eye glasses

Salvino D’Armate is credited with inventing the first wearable eye glasses.

1590

Early microscope

Zacharias Janssen and his son Hans place multiple lenses in a tube. They observe that viewed objects in front of the tube appear greatly enlarged. This is a forerunner of the compound microscope and the telescope.

1609

Compound microscope

Galileo Galilei develops a compound microscope with a convex and a concave lens.

1625

First use of term ‘microscope’

Giovanni Faber coins the name ‘microscope’ for Galileo Galilei’s compound microscope.

1665

First use of term ‘cells’

English physicist Robert Hooke publishesMicrographia, in which he coins the term ‘cells’ when describing tissue. The book includes drawings of hairs on a nettle and the honeycomb structure of cork. He uses a simple, single-lens microscope illuminated by a candle.

1676

Living cells first seen

Antonie van Leeuwenhoek builds a simple microscope with one lens to examine blood, yeast and insects. He is the first to describe cells and bacteria. He invents new methods for making lenses that allow for magnifications of up to 270 times.

1830

Spherical aberration solved

Joseph Jackson Lister reduces spherical aberration (which produces imperfect images) by using several weak lenses together at certain distances to give good magnification without blurring the image.

1874

Abbe equation

Ernst Abbe writes a mathematical formula that correlates resolving power to the wavelength of light. Abbe’s formula makes it possible to calculate the theoretical maximum resolution of a microscope.

1931

Transmission electron microscope

Ernst Ruska and Max Knoll design and build the first transmission electron microscope (TEM), based on an idea of Leo Szilard. The electron microscope depends on electrons, not light, to view an object. Modern TEMs can visualise objects as small as the diameter of an atom.

1932

Phase contrast microscope

Frits Zernike develops phase contrast illumination, which allows the imaging of transparent samples. By using interference rather than absorption of light, transparent samples, such as cells, can be imaged without having to use staining techniques.

1942

Scanning electron microscope

Ernst Ruska builds the first scanning electron microscope (SEM), which transmits a beam of electrons across the surface of a specimen.

1957

Confocal imaging principle

Marvin Minsky patents the principle of confocal imaging. Using a scanning point of light, confocal microscopy gives slightly higher resolution than conventional light microscopy and makes it easier to view ‘virtual slices’ through a thick specimen.

1962

Green fluorescent protein (GFP) discovered

Osamu Shimomura, Frank Johnson and Yo Saiga discover green fluorescent protein (GFP) in the jellyfishAequorea victoria. GFP fluoresces bright green when exposed to blue light.

1972

First CAT scanner

Godfrey Hounsfield and Allan Cormack develop the computerised axial tomography (CAT) scanner. With the help of a computer, the device combines many X-ray images to generate cross-sectional views as well as three-dimensional images of internal organs and structures.

1973

Electron backscatter patterns observed

John Venables and CJ Harland observe electron backscatter patterns (EBSP) in the scanning electron microscope. EBSP provide quantitative microstructural information about the crystallographic nature of metals, minerals, semiconductors and ceramics.

1978

Confocal laser scanning microscope

Thomas and Christoph Cremer develop the first practical confocal laser scanning microscope, which scans an object using a focused laser beam.

1981

Scanning tunnelling microscope

Gerd Binnig and Heinrich Rohrer invent the scanning tunnelling microscope (STM). The STM ‘sees’ by measuring interactions between atoms, rather than by using light or electrons. It can visualise individual atoms within materials.

1986

Nobel Prize for microscopy

The Nobel Prize in Physics is awarded jointly to Ernst Ruska (for his work on the electron microscope) and to Gerd Binnig and Heinrich Rohrer (for the scanning tunnelling microscope).

1992

Green fluorescent protein (GFP) cloned

Douglas Prasher reports the cloning of GFP. This opens the way to widespread use of GFP and its derivatives as labels for fluorescence microscopy (particularly confocal laser scanning fluorescence microscopy).

1993–1996

Super-resolution microscopy

Stefan Hell pioneers a new optical microscope technology that allows the capture of images with a higher resolution than was previously thought possible. This results in a wide array of high-resolution optical methodologies, collectively termed super-resolution microscopy.

2010

Atoms of a virus seen

Researchers at UCLA use a cryoelectron microscope to see the atoms of a virus.

2014

Chemistry Nobel prize for super microscopes

Nobel Prize in Chemistry awarded to Eric Betzig, Stefan Hell and William Moerner for the development of super-resolved fluorescence microscopy which allows microscopes to now ‘see’ matter smaller than 0.2 micrometres.

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